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TECHNICAL PAPERS

Hydrodynamic Focusing for a Micromachined Flow Cytometer

[+] Author and Article Information
Gwo-Bin Lee

Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan, Republic of China, 701e-mail: gwobin@mail.ncku.edu.tr

Chen-I Hung, Bin-Jo Ke

Department of Mechanical Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China, 701

Guan-Ruey Huang, Bao-Herng Hwei, Hui-Fang Lai

Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan, Republic of China, 701

J. Fluids Eng. 123(3), 672-679 (Apr 18, 2001) (8 pages) doi:10.1115/1.1385514 History: Received September 25, 2000; Revised April 18, 2001
Copyright © 2001 by ASME
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References

Figures

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Schematic representation of a conventional flow cytometer. Center flow is focused hydrodynamically into a single cell stream due to sheath flows
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Schematic representation of the micro flow cytometer (a), and close-up schematic of the convergent nozzle
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Fabrication process for quartz master templates. Microfluidic devices on the quartz substrate were formed by using wet etching (buffered oxide etchant, BOE, 6:1) with Cr as etch masks
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Schematic diagram of hot embossing methods. A hot embossing machine was used to apply uniform and reproducible pressure on the surfaces. The entire devices were heated above transition temperature of the plastics for at least 10 minutes
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Picture of a microfabricated flow cytometer. It was fabricated on PMMA substrates using hot embossing methods
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Cross-sectional geometry of the micro channels. Note that 10 sets of data are plotted on the same figure along with one from a quartz template
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The coordination of potential flow theory showing the fluid passing through the cross section (A) in the distance (s) is focused to a smaller cross section (a) inside a tube 15
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Schematic representation of a micromachined flow cytometer. Note that inner and outer nozzles have a convergent profile
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Variation of the width of the focused stream along with axial location for flows with different relative flow rates. Note that the focused width (d) is normalized by a reference width (d0), which is the width measured at the exit of the inner nozzle
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The variation of the width of the focused stream along with relative flow rates while measured at the exit of the flow cytometer
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The variation of the focused length along with the relative sheath and sample flow rates
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Focused sample stream inside a flow cytometer. Hydrodynamic focsuing is verified with the use of microscopic visualization of water sheath flows and dye-containing sample flow
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The variation of the width of the focused stream along with relative flow rates
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The variation of the focused length along with the relative sheath and sample flow rates

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