In this paper, the flow and mass transfer of a two-dimensional unsteady stagnation-point flow over a moving wall, considering the coupled blowing effect from mass transfer, is studied. Similarity equations are derived and solved in a closed form. The flow solution is an exact solution to the two-dimensional unsteady Navier–Stokes equations. An analytical solution of the boundary layer mass transfer equation is obtained together with the momentum solution. The examples demonstrate the significant impacts of the blowing effects on the flow and mass transfer characteristics. A higher blowing parameter results in a lower wall stress and thicker boundary layers with less mass transfer flux at the wall. The higher wall moving parameters produce higher mass transfer flux and blowing velocity. The Schmidt parameters generate a local maximum for the mass transfer flux and blowing velocity under given wall moving and blowing parameters.