An experimental investigation has been conducted to identify the effects of pressure gradient and surface roughness on turbulent boundary layers. In Part II, smooth- and rough-surface turbulent boundary layers with and without adverse pressure gradient (APG) are presented at a fixed Reynolds number (based on the length of flat plate) of 900,000. Flat-plate boundary layer measurements have been conducted using a single-sensor, hot-wire probe. For smooth surfaces, compared to the zero pressure gradient (ZPG) boundary layer, the APG boundary layer has a higher mean velocity defect throughout the boundary layer and lower friction coefficient. APG decreases the streamwise normal Reynolds stress for y less than 0.4 times the boundary layer thickness and increases it slightly in the outer region. For rough surfaces, APG reduces the roughness effects of increasing the mean velocity defect and normal Reynolds stress for y less than 23 and 28 times the average roughness height, respectively. Consistently, for the same roughness, APG decreases the integrated streamwise turbulent kinetic energy. APG also decreases the roughness effect on the friction coefficient, roughness Reynolds number, and roughness shift. Compared to the ZPG boundary layers, the roughness effects on integral boundary layer parameters—boundary layer thickness and momentum thickness—are weaker under APG. Thus, contrary to the favorable pressure gradient (FPG) in part I, APG reduces the roughness effects on turbulent boundary layers.