Gold nanoparticles have been found to greatly enhance the polymerase chain reaction (PCR) specificity and yield in recent studies. However, the underlying mechanism is still unclear, though different hypotheses have already been proposed. In this study, a mass-action based model has been developed to investigate the effect of Au nanoparticles on the two-round PCR results. The great affinity of Au nanoparticles to the single-stranded DNA is taken into consideration. Each nanoparticle is treated as a bioreactor and/or a selector, around which, reaction equations are coupled to simulate the particle effect, and to investigate the key parameters that might influence such an effect. It is assumed that there exists a competing mechanism between the specific and nonspecific bindings, both in the solution and on the particle surface during the reactions. The numerical predictions accord well to the experimental results, and can be used to explain the Au nanoparticles’ effect on the enhancement of the PCR specificity and efficiency.
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e-mail: lisaxu@sjtu.edu.cn
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July 2009
Technical Briefs
Numerical Simulation of Au Nanoparticles Effect on the PCR Process
Chao Chen,
Chao Chen
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
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Aili Zhang,
Aili Zhang
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
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Xiaodong Zhang,
Xiaodong Zhang
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
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Jun Hu,
Jun Hu
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
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Lisa X. Xu
Lisa X. Xu
Professor
Department of Biomedical Engineering,
e-mail: lisaxu@sjtu.edu.cn
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China; Med-X Research Institute, Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
Search for other works by this author on:
Chao Chen
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
Aili Zhang
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
Xiaodong Zhang
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
Jun Hu
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China
Lisa X. Xu
Professor
Department of Biomedical Engineering,
Shanghai Jiao Tong University
, Shanghai 200240, P.R. China; Med-X Research Institute, Shanghai Jiao Tong University
, Shanghai 200240, P.R. Chinae-mail: lisaxu@sjtu.edu.cn
J Biomech Eng. Jul 2009, 131(7): 074512 (7 pages)
Published Online: June 12, 2009
Article history
Received:
September 12, 2008
Revised:
May 3, 2009
Published:
June 12, 2009
Citation
Chen, C., Zhang, A., Zhang, X., Hu, J., and Xu, L. X. (June 12, 2009). "Numerical Simulation of Au Nanoparticles Effect on the PCR Process." ASME. J Biomech Eng. July 2009; 131(7): 074512. https://doi.org/10.1115/1.3147746
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