The use of surgically implanted or nonsurgically inserted medical devices has received an escalating interest in modern medical practices. Upon implantation or insertion into patient’s body for exerting the intended purpose like salvage of normal functions of vital organs, the medical devices are unfortunately becoming the sites of competition between host cell integration and microbial adhesion. To control microbial colonization and subsequent biofilm formation onto the medical devices, different approaches either to enhance the efficiency of certain antimicrobial agents or to disrupt the basic physiology of the pathogenic micro-organisms, including novel small molecules and antipathogenic drugs, are being explored. In addition, the various lipid- and polymer-based drug delivery carriers are also investigated for applying antibiofilm coating onto the medical devices especially over catheters. The major objectives of this paper are as follows: (1) to synthesize magnesium fluoride (MgF2) nanoparticles; (2) to prepare MgF2 nanoparticle-stabilized oil-in-water (o/w) nanosized emulsion; (3) to coat biomaterial surfaces (glass coupons) with MgF2 nanoparticles, and MgF2 nanoparticle-stabilized emulsion; (4) to challenge the coated and uncoated glass surfaces with fresh bacterial cultures (i.e., Escherichia coli and Staphylococcus aureus) in 24-well plate over 18 h for biofilm formation; and (5) to compare the efficacy of emulsion-coated and emulsion-uncoated glass coupons in restricting the bacterial growth and biofilm formation.